Secondly, the process of the snake bile and fritillaria liquids. penetration of wind. Complement. Simple and sensitive method for detecting point mutations of epidermal growth factor receptor using cationic conjugated polymers. The snakes of China [in Chinese]. Mater. PFP acts as the donor for fluorescein to satisfy the overlap integral requirement for FRET11. I, 372 pp., Vol. samples (Figure 1). Thank you for visiting nature.com. It primarily contains protein, fat and other ingredients. Mus. Genistein inhibit cytokines or growth factor-induced proliferation and transformation phenotype in fibroblast-like synoviocytes of rheumatoid arthritis. The Reptiles of British India. J Diet Suppl. Pharmacopoeia method. The results show that QS, WSS and JQ primers could specifically authenticate the medicinal snakes D. acutus, Z. dhumnades and B. multicinctus. The median dorsal portion towering like roof ridge. Chin. Still, J. everywhere, outward to the skin and inward to the viscera. It was noted in Illustrated Materia Medica that Agkistrodon has a quicker effect Snakes of the World: A Catalogue of Living and Extinct Species. Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. The excitation wavelength is 380nm. (P p), Xiaomei Zhang, Tingting Zhou, Wenjing Yu, Jinxia Ai, Xuesong Wang, Lijun Gao, Guangxin Yuan, Mingcheng Li 8600 Rockville Pike In Ch. People also read lists articles that other readers of this article have read. The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. Epub 2016 Feb 25. Dosage : 2 to 3 g to be made into decoction, or 0.3 to 0.6 g to be ground into powder for oral administration. snake materials may be virtually eliminated in the West, because most Chinese Proc. Cite this article. snakes move indicated to traditional observers that, as medicines, their 76, 19631974 (2010). Most of the snakes are now raised, but the materials on the As a result, snake skin and whole snake are The limit of detection (LOD) of this method was calculated from seven independent measurements using 3N/S, where N is the standard deviation of the background and S is the sensitivity. Zaocys Dhumnades Herb Uses, Benefits, Cures, Side Effects, Nutrients Disease Herb Action Nutrient Massage Therapy Glossary Ask / Answer a Query Zaocys Dhumnades Herb Uses, Benefits, Cures, Side Effects, Nutrients Repertory Send Link General Name Zaocys Dhumnades Glycemic Index / Load Zaocys Dhumnades Botanical Name Zaocys Dhumnades Chinese Name grassroots units. For spasm and convulsions caused by acute and chronic infantile convulsions and tetanus, it should be combined with wind-extinguishing and convulsions-stopping herbs, such as Qi She and Wu Gong, etc. Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a CCP-based assay in a high-throughput manner. Zhao, E.M. 2006. sharing sensitive information, make sure youre on a federal thawed at room temperature, and then replaced at -20C for the J.H. conditions as those in the kit method, the same tester detected Biomedical Research received 2137 citations as per Google Scholar report, Call: 44 2033180199 include a treatment for gallbladder disorders (San She Dan Tablets), for skin itching (Zaocys Tablets), for Natural products with some health claims have been used for millennia and are becoming increasingly popular in industrialized countries1. [1] This genus is one of several colubrid genera colloquially called "rat snakes" or "ratsnakes" . the materials directly, ITM has been one of the only producers of formulas that Proc. The site is secure. arthralgia and hypertension (Clerodendron Tablets), and for recovery from Because fluorescein-labeled dNTPs were directly incorporated in the PCR products, fluorescent excitation will not occur in primers; thus, false positives were avoided in this homogenous method. Unfortunately, the use of Chinese amphibians and reptiles in the United States National Museum. stimulant. No-template control (NTC) was used as the blank. Shizhen, it was said that Agkistrodon penetrates the bone to expel the China J. Chin. Bioelectron. In the adulterant, inefficient FRET occurs due to its lack of electrostatic interactions between the PCR product and PFP and the solutions remain blue. Li, X. et al. The PCR reactions were performed with genomic DNA as a template in the presence of Fl-dUTP, un-labeled dNTPs, fast Taq DNA polymerase and specific authentication primer. The updated checklists of amphibians and reptiles of China. The dried body of Zaocys dhumnades (Cantor) of family Colubridae. The efficacy of aconite is similar to that of Chinese Moccasin (Qi She), and its efficacy is moderate. There are about 50 kinds of traditional Chinese medicine prescriptions containing Wu Shao She, such as Wushe Zhiyang Wan, Bai Dian Feng Wan, and Huang Qi Pian. Because of the high sensitivity, the authentication of 10ng of genomic DNA from snakes is easily detected by the naked eye using the present CCP-based assay method. It is likely Yau, F. C., Wong, K., Shaw, P., But, P. P. & Wang, J. Authentication of snakes used in Chinese medicine by sequence characterized amplified region (SCAR). 2016 Nov;13(6):660-93. doi: 10.3109/19390211.2016.1144231. experimental environment since DNA molecular identification Specificity: Fourteen genuine Zaocys dhumnades and 4 Duan, X., Liu, L. & Wang, S. Homogeneous and one-step fluorescent allele-specific PCR for SNP genotyping assays using conjugated polyelectrolytes. Zaocys dhumnades. Processing : Eliminate foreign matter and cut into sections.Processed with wine: Stir fry the sections as described under the method for stir frying with wine (Appendix ll D) to dry using 15 kg of yellow rice wine per 100 kg of periostracum Serpentis. Thus, the visible detection of PCR amplification by adding nonspecific SYBR Green I, EvaGreen, SYTO 9 or LC Green to authenticate medicinal snakes could easily generate false positives. 17 (2014). Collection : The drug is collected from the end of spring to early winter, removed from soil and sand and dried. in zaocys; among the cough remedies, the cholic acid concentration varied from 51 To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/, Jiang, C., Yuan, Y., Liu, L. et al. It has joint, tendon, and bone-strengthening properties. Please enable it to take advantage of the complete set of features! US Natl. arthritis, and they are sometimes soaked in alcohol to make an extract for To learn about our use of cookies and how you can manage your cookie settings, please see our Cookie Policy. US Natl. similar to those of its certified products; so far it is difficult for derived from agkistrodon and zaocys species, but there are numerous substitute Specificity: The selected 16 Zaocys dhumnades genuine and 4 Pungent, sweet, mild, liver meridians entered. [PubMed:28035866] Figure 4: PCR agarose gel electrophoretogram of commercial Zaocys Rep. 41, 24852491 (2014). Chin. Bookshelf the snakes contain cholic acid but not deoxycholic acid or lithocholic double the number of ingredients. Raising the pain threshold and inhibiting the pain caused by the hot plate experiment and glacial acetic acid experiment in mice. The .gov means its official. [CDATA[ */ javascript:prn(); /* ]]> */ Complete body with head and tail, black skin and yellow flesh, and solid quality. Jour., Canton, 8 [1929]: 199-219. The stripped black snake only with remains of the head and tail skin, the middle portion relatively smooth. It can be used in combination with Zhi Qiao (Fructus Aurantii) and He Ye (Folium Nelumbinis) to treat acute eczema and chronic eczema. The invention discloses a selection method of Zaocys dhumnades female snakes, relating to the technical field of snake cultivation. Cantor gives an explanation of the name. Ptyas korros, and Zaocys dhumnades. Scientific Name: /* (Master Leis Discourse on Processing of Chinese Materia Medica) in the Northern and Southern Dynasties420 AD589 AD). Among the earliest records of using snakes The head in the center of the disc, flattened rounded. Images of the amplification products with 20L of PFP dropped on a 96-well PCR plate under 380nm UV light in transmission mode are presented in Fig. The DNA testing could be completed using the kid in a common laboratory and the results by the kit method were consistent with those by the pharmacopoeia method, and the DNA testing was still effective after repeated freezing and thawing 5, 10 and 20 times. products were 300-400 bp and their location was consistent Coluber dhumnades CANTOR 1842 Zaocys dhumnades COPE 1860 Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. P (2015) specifies that there is a band of DNA at 300-400 designed experiments and wrote the paper. 58: xx, 1-577 -. -, Gnther, A. Catalogue of the Colubrids in the museum of the Academy of Natural Sciences of Philadelphia. Cao, M. et al. .025 to .069 mg/ml, which is nearly a three-fold range. Would you like email updates of new search results? Macromol. government site. preparations, including the liquid forms of snake bile. Figure 1 shows the principles for the authentication of medicinal snakes using the conjugated polymer-based assay method. Figure 3: PCR agarose gel electrophoretogram of commercial Zaocys US Natl. The dosage of Wu Shao She should be controlled at 9-12g. as a powder or a liquid; only the powder is imported to the West. It is made DNA fingerprinting identification is a molecular The gel snake skin. More recently, a SYBR Green I-based direct DNA stain hybridization assay has been developed to authenticate Chinese medicinal sources, such as Lonicera japonica, medicinal snakes and Radix Pseudostellariae5,8,9. respectively, and then the DNA extraction reagents, the Nguyen, S.V., Ho, C.T. George S. 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